Johnson State College competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Basketball | 14 | 13 |
| Golf | 6 | 0 |
| Lacrosse | 19 | 14 |
| Soccer | 25 | 23 |
| Softball | 0 | 14 |
| Tennis | 8 | 11 |
| Track and Field, Outdoor | 8 | 8 |
| Track and Field, X-Country | 9 | 8 |
| Volleyball | 0 | 9 |