Limestone College competes in NCAA Division II (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 94 | 0 |
| Basketball | 15 | 23 |
| Field Hockey | 0 | 37 |
| Football | 166 | 0 |
| Golf | 16 | 10 |
| Lacrosse | 71 | 32 |
| Soccer | 40 | 39 |
| Softball | 0 | 25 |
| Swimming | 23 | 21 |
| Tennis | 9 | 9 |
| Track and Field, Outdoor | 45 | 30 |
| Track and Field, X-Country | 15 | 11 |
| Volleyball | 15 | 18 |
| Wrestling | 57 | 0 |