Wilkes University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 42 | 0 |
| Basketball | 16 | 17 |
| Field Hockey | 0 | 21 |
| Football | 87 | 0 |
| Golf | 8 | 7 |
| Lacrosse | 22 | 19 |
| Soccer | 25 | 30 |
| Softball | 0 | 14 |
| Swimming | 13 | 16 |
| Tennis | 14 | 14 |
| Track and Field, X-Country | 11 | 8 |
| Volleyball | 0 | 14 |
| Wrestling | 33 | 0 |