Waynesburg University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 37 | 0 |
| Basketball | 16 | 19 |
| Football | 113 | 0 |
| Golf | 5 | 5 |
| Lacrosse | 0 | 15 |
| Soccer | 22 | 21 |
| Softball | 0 | 14 |
| Tennis | 6 | 12 |
| Track and Field, Indoor | 27 | 22 |
| Track and Field, Outdoor | 27 | 22 |
| Track and Field, X-Country | 14 | 20 |
| Volleyball | 0 | 22 |
| Wrestling | 16 | 0 |