Messiah College competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 40 | 0 |
| Basketball | 17 | 18 |
| Field Hockey | 0 | 24 |
| Golf | 5 | 0 |
| Lacrosse | 37 | 26 |
| Soccer | 25 | 28 |
| Softball | 0 | 24 |
| Swimming | 17 | 22 |
| Tennis | 9 | 11 |
| Track and Field, Indoor | 57 | 49 |
| Track and Field, Outdoor | 56 | 45 |
| Track and Field, X-Country | 29 | 17 |
| Volleyball | 0 | 17 |
| Wrestling | 33 | 0 |