Holy Family University competes in NCAA Division II (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Basketball | 14 | 13 |
| Lacrosse | 0 | 15 |
| Soccer | 23 | 21 |
| Softball | 0 | 17 |
| Tennis | 0 | 5 |
| Track and Field, Indoor | 10 | 13 |
| Track and Field, Outdoor | 9 | 14 |
| Track and Field, X-Country | 11 | 10 |
| Volleyball | 0 | 13 |