DeSales University competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 36 | 0 |
| Basketball | 17 | 16 |
| Field Hockey | 0 | 17 |
| Golf | 10 | 0 |
| Lacrosse | 29 | 0 |
| Soccer | 29 | 26 |
| Softball | 0 | 18 |
| Track and Field, Outdoor | 38 | 23 |
| Track and Field, X-Country | 19 | 13 |
| Volleyball | 0 | 13 |