Wittenberg University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 32 | 0 |
| Basketball | 24 | 21 |
| Field Hockey | 0 | 26 |
| Football | 122 | 0 |
| Golf | 11 | 7 |
| Lacrosse | 38 | 18 |
| Soccer | 34 | 28 |
| Softball | 0 | 19 |
| Swimming and Diving | 19 | 31 |
| Tennis | 14 | 9 |
| Track and Field, Indoor | 49 | 34 |
| Track and Field, Outdoor | 49 | 34 |
| Track and Field, X-Country | 17 | 22 |
| Volleyball | 17 | 21 |