Ohio Northern University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 38 | 0 |
| Basketball | 32 | 19 |
| Football | 147 | 0 |
| Golf | 12 | 11 |
| Lacrosse | 34 | 19 |
| Soccer | 40 | 30 |
| Softball | 0 | 19 |
| Swimming and Diving | 24 | 21 |
| Tennis | 8 | 7 |
| Track and Field, Indoor | 56 | 51 |
| Track and Field, Outdoor | 53 | 47 |
| Track and Field, X-Country | 35 | 24 |
| Volleyball | 0 | 15 |
| Wrestling | 25 | 0 |