Defiance College competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 38 | 0 |
| Basketball | 16 | 15 |
| Football | 91 | 0 |
| Golf | 8 | 5 |
| Lacrosse | 16 | 0 |
| Soccer | 26 | 16 |
| Softball | 0 | 33 |
| Tennis | 9 | 10 |
| Track and Field, Indoor | 26 | 14 |
| Track and Field, Outdoor | 27 | 14 |
| Track and Field, X-Country | 8 | 5 |
| Volleyball | 0 | 13 |