Guilford College competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 46 | 0 |
| Basketball | 26 | 14 |
| All Track Combined | 47 | 48 |
| Football | 123 | 0 |
| Golf | 9 | 0 |
| Lacrosse | 35 | 17 |
| Soccer | 29 | 21 |
| Softball | 0 | 29 |
| Swimming | 0 | 7 |
| Tennis | 11 | 8 |
| Volleyball | 0 | 11 |