Saint John Fisher College competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 41 | 0 |
| Basketball | 31 | 13 |
| Field Hockey | 0 | 24 |
| Football | 124 | 0 |
| Golf | 14 | 7 |
| Lacrosse | 47 | 29 |
| Rowing | 11 | 13 |
| Soccer | 44 | 45 |
| Softball | 0 | 19 |
| Tennis | 10 | 7 |
| Track and Field, Indoor | 33 | 29 |
| Track and Field, Outdoor | 27 | 24 |
| Track and Field, X-Country | 14 | 10 |
| Volleyball | 0 | 14 |