Niagara University competes in NCAA Division I-AAA.
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 31 | 0 |
| Basketball | 15 | 12 |
| Golf | 10 | 6 |
| Ice Hockey | 30 | 0 |
| Lacrosse | 0 | 28 |
| Soccer | 25 | 24 |
| Softball | 0 | 21 |
| Swimming and Diving | 19 | 18 |
| Tennis | 7 | 7 |
| Track and Field, Outdoor | 0 | 33 |
| Track and Field, X-Country | 9 | 14 |
| Volleyball | 0 | 12 |