D'Youville College competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 20 | 0 |
| Basketball | 15 | 13 |
| Golf | 9 | 0 |
| Lacrosse | 0 | 13 |
| Rowing | 0 | 11 |
| Soccer | 26 | 18 |
| Softball | 0 | 16 |
| Tennis | 11 | 14 |
| Track and Field, X-Country | 7 | 7 |
| Volleyball | 11 | 13 |