Nebraska Wesleyan University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 31 | 0 |
| Basketball | 33 | 16 |
| All Track Combined | 144 | 88 |
| Football | 117 | 0 |
| Golf | 11 | 9 |
| Soccer | 22 | 24 |
| Softball | 0 | 21 |
| Swimming | 15 | 9 |
| Tennis | 13 | 10 |
| Volleyball | 0 | 23 |