Missouri Valley College competes in NAIA Division I.
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 28 | 0 |
| Basketball | 15 | 15 |
| Football | 65 | 0 |
| Golf | 12 | 6 |
| Lacrosse | 41 | 14 |
| Soccer | 26 | 21 |
| Softball | 0 | 22 |
| Tennis | 13 | 10 |
| Track and Field, Indoor | 24 | 9 |
| Track and Field, Outdoor | 25 | 11 |
| Track and Field, X-Country | 12 | 8 |
| Volleyball | 17 | 21 |
| Wrestling | 17 | 14 |
| Rodeo | 27 | 23 |