Lesley University competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 26 | 0 |
| Basketball | 12 | 10 |
| Soccer | 24 | 25 |
| Softball | 0 | 15 |
| Tennis | 7 | 7 |
| Track and Field, Indoor | 7 | 14 |
| Track and Field, Outdoor | 7 | 14 |
| Track and Field, X-Country | 5 | 10 |
| Volleyball | 10 | 12 |