Transylvania University competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 35 | 0 |
| Basketball | 18 | 17 |
| Field Hockey | 0 | 14 |
| Golf | 14 | 6 |
| Lacrosse | 32 | 17 |
| Soccer | 36 | 22 |
| Softball | 0 | 19 |
| Swimming | 9 | 15 |
| Tennis | 15 | 12 |
| Track and Field, Indoor | 15 | 18 |
| Track and Field, Outdoor | 14 | 18 |
| Track and Field, X-Country | 9 | 5 |
| Volleyball | 0 | 14 |