Spalding University competes in NCAA Division III (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 34 | 0 |
| Basketball | 19 | 11 |
| Golf | 8 | 6 |
| Soccer | 27 | 22 |
| Softball | 0 | 12 |
| Track and Field, Indoor | 23 | 18 |
| Track and Field, Outdoor | 23 | 13 |
| Track and Field, X-Country | 14 | 12 |
| Volleyball | 0 | 14 |
| Bowling | 0 | 7 |