Saint Ambrose University competes in NAIA Division II.
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 47 | 0 |
| Basketball | 58 | 15 |
| Football | 182 | 0 |
| Golf | 26 | 7 |
| Lacrosse | 30 | 0 |
| Soccer | 36 | 41 |
| Softball | 0 | 26 |
| Tennis | 7 | 12 |
| Track and Field, Outdoor | 41 | 35 |
| Track and Field, X-Country | 12 | 14 |
| Volleyball | 23 | 25 |
| Bowling | 24 | 23 |