University of Dubuque competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 40 | 0 |
| Basketball | 30 | 25 |
| Football | 156 | 0 |
| Golf | 10 | 7 |
| Lacrosse | 17 | 22 |
| Soccer | 50 | 33 |
| Softball | 0 | 25 |
| Tennis | 7 | 7 |
| Track and Field, Indoor | 64 | 37 |
| Track and Field, Outdoor | 42 | 34 |
| Track and Field, X-Country | 19 | 18 |
| Volleyball | 0 | 24 |
| Wrestling | 45 | 0 |