Trine University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 54 | 0 |
| Basketball | 29 | 25 |
| Football | 175 | 0 |
| Golf | 30 | 13 |
| Lacrosse | 37 | 17 |
| Soccer | 48 | 25 |
| Softball | 0 | 21 |
| Tennis | 14 | 12 |
| Track and Field, Indoor | 53 | 16 |
| Track and Field, Outdoor | 53 | 16 |
| Track and Field, X-Country | 27 | 8 |
| Volleyball | 0 | 22 |
| Wrestling | 16 | 0 |