Millikin University competes in NCAA Division III (with football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 47 | 0 |
| Basketball | 21 | 22 |
| Football | 113 | 0 |
| Golf | 15 | 5 |
| Soccer | 36 | 31 |
| Softball | 0 | 21 |
| Swimming | 6 | 15 |
| Tennis | 12 | 8 |
| Track and Field, Indoor | 25 | 23 |
| Track and Field, Outdoor | 26 | 21 |
| Track and Field, X-Country | 11 | 12 |
| Volleyball | 0 | 24 |
| Wrestling | 41 | 0 |