Saint Leo University competes in NCAA Division II (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 35 | 0 |
| Basketball | 15 | 15 |
| Golf | 13 | 10 |
| Lacrosse | 52 | 26 |
| Soccer | 27 | 25 |
| Softball | 0 | 20 |
| Swimming | 14 | 18 |
| Tennis | 10 | 8 |
| Track and Field, Outdoor | 16 | 17 |
| Track and Field, X-Country | 18 | 17 |
| Volleyball | 0 | 14 |