Jacksonville University competes in NCAA Division I-AA.
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 37 | 0 |
| Basketball | 16 | 13 |
| All Track Combined | 0 | 59 |
| Football | 117 | 0 |
| Golf | 9 | 8 |
| Lacrosse | 45 | 37 |
| Rowing | 23 | 35 |
| Soccer | 30 | 24 |
| Softball | 0 | 19 |
| Track and Field, X-Country | 9 | 0 |
| Volleyball | 0 | 14 |
| Beach Volleyball | 0 | 17 |