Florida Southern College competes in NCAA Division II (without football).
| Name | Participants | |
|---|---|---|
| Men | Women | |
| Baseball | 37 | 0 |
| Basketball | 15 | 12 |
| All Track Combined | 33 | 37 |
| Golf | 10 | 11 |
| Lacrosse | 41 | 32 |
| Soccer | 25 | 32 |
| Softball | 0 | 19 |
| Swimming | 23 | 18 |
| Tennis | 16 | 11 |
| Volleyball | 0 | 18 |